New Liposome developed by click chemistry
ScreenFect™mRNA is a transfection reagent consisting of a new cationic liposome by click chemistry screening system*1. ScreenFect™mRNA is suitable for transfection into eukaryotic cells (HeLa, HEK293, MDF cells, etc.) without the replacement of culture medium after transfection because it is not included hazard compounds. ScreenFect™mRNA is used for mRNA transfection procedures.
*1: Biomaterials, Nov.; 33(32): 8160-76, 2012.
- Direct transfection of mRNA
- Long RNA transfection
- Establish iPS cell line without virus vector
Application data
1.GFP mRNA transfection into HEK293 cells
Transfection conditions of ScreenFect™mRNA
- ・Cell line: HEK293
- ・Protocol: 1-Step method (See below), no-medium replacement
- ・Well format: 96 well plate
- ・Amount of transfection reagent: 0.3 μL/well
- ・Amount of RNA: GFP mRNA [TriLink #L-6101] 75 ng/well
- ・Detection time: 24 hours after the transfection
- ・Detector: Cytation3 microscope [BioTek]
2. EGFP mRNA transfection into HeLa cell (with SFA P-reagent)
Transfection conditions of ScreenFect™mRNA (SF mRNA), SF mRNA + SFA P-reagent
- [Cell number] 0.7x105 cells/well
- [Amount of mRNA] 0.1μg/assay
- [Ratio] mRNA（μg）：ScreenFect™mRNA reagent（μl）= 1：4
- [Amount of SFA P-reagent] 0.2μl/well
mRNA（μg）：SFA P-reagent（μl） = 1：2 - [Well format] 24 well plates
- [Detection time] 48 hours after the transfection
ScreenFect™mRNA transfection conditions
| mRNA transfection (/well) Standard Protocol | ||||||
|---|---|---|---|---|---|---|
| Plate size | Surface area | Medium volume | Total volume mRNA-lipid complex | Rate of mRNA : Transfection Reagent | mRNA / Dilution Buffer | Transfection Reagent / Dilution Buffer |
| 96 wells | 0.3cm2 | 100μl | 10μl | 1:3 | 50ng / 5μl | 0.15μl / 5μl |
| 1:4 | 0.2μl / 5μl | |||||
| 24 wells | 2cm2 | 500μl | 50μl | 1:3 | 250ng / 25μl | 0.75μl / 25μl |
| 1:4 | 1.0μl / 25μl | |||||
| 12 wells | 4cm2 | 1000μl | 100μl | 1:3 | 500ng / 50μl | 1.5μl / 50μl |
| 1:4 | 2.0μl / 50μl | |||||
| 6 wells | 10cm2 | 2000μl | 250μl | 1:3 | 1250ng / 125μl | 3.75μl / 125μl |
| 1:4 | 5.0μl / 125μl | |||||
Outline of ScreenFect™mRNA protocol
The ideal mixing ratio of mRNA and transfection reagent varies depending on the type of cells.
We will recommend you to examine several ratios and choose the best one.
ScreenFect™mRNA product information
Now on sales campaign! We will offer special price during the campaign.
[Term of campaign : November 11, 2016 to February 28, 2017 ] For further information, inquires to our distributors.
| Code No. | Product name | ScreenFect™mRNA Transfection Reagent | Dilution Buffer for ScreenFect™mRNA | Price (CNY) |
|---|---|---|---|---|
| 293-75901 | ScreenFect™mRNA |
0.2ml | 10ml | 1,350 |
| 299-75903 | ScreenFect™mRNA |
1ml | 50ml | 5,270 |
| 297-75904 | ScreenFect™mRNA |
1ml×5 | 50ml×5 | 24,120 |