Coating

Collagen, Research Reagent (Liquid)

Characteristics of Collagen Classified Based on Its Molecular Species and Extraction Methods

◆Collagen type I
Type I collagen is the major fibrous protein in the extracellular matrix. It can be found in most organs, but is particularly abundant in bone, tendon, and dermis.

Acid Soluble Collagen(ASC)
・Due to its high gel strength, this collagen preparation is ideal for both “On-Gel” and “In-Gel (Gel-Embedded)” cultures. 
・Filtered through a 0.45 μm pore size membrane.

Pepsin Solubilized Collagen (PSC)
・ Atelocollagen prepared by enzymatically removing telopeptides. 
・ This form of collagen has high cellular adhesiveness, making it perfect for coating cell culture vessel surfaces.
・ Filtered through a 0.45 μm pore size membrane.

◆Collagen type Ⅲ
This is a fibrillar collagen abundant in tissues with high elasticity, such as juvenile skin tissues and the arterial tunica media where smooth muscle cells populate. Type III collagen is also expressed during embryogenesis and wound healing.

・Atelocollagen prepared by enzymatically removing telopeptides. 
・Filtered through a 0.45 μm pore size membrane.

◆Collagen type Ⅳ
Type IV collagen is one of the major components of basement membranes. It polymerizes to form a mesh-like network in the basal lamina.

・Acid-extracted native collagen. 
・This form of collagen has high cellular adhesiveness . It is suitable for collagen-gel cultures and collagen-coating of culture vessel surfaces.

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Nitta Gelatin's collagen- and gelatin-based reagents are trusted products with proven performance that have long been used by many researchers. Their collagen products have superior optical clarity even after gel formation is completed. This provides an opportunity to accurately observe biological samples embedded in collagen gel matrices.

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This product, called Cellcampus®, is a preparation of type I collagen derived from the scales of farm-raised tilapia, a tropical fish. Cellcampus® is manufactured by Taki Chemical Co., Ltd. in Japan. 00539_img02.jpg

There are no known viruses transmitted from tilapia to humans. Furthermore, tropical fish collagens show higher denaturation temperatures, as compared with collagens derived from non-tropical fish. Thus, Cellcampus® is perfect for tissue and cell culture applications in the field of regenerative medicine. 00539_img03.jpg
It is well documented that tilapia scale collagen exhibits an excellent fibril-forming ability. Moreover, this collagen can enhance cell proliferation. Therefore, Cellcampus® is also ideal for general cell culture protocols.

Laminins are a major basement membrane component of most animal tissues. . In cell culture, laminins have been used for coating the surfaces of cell culture vessels. It is widely known that laminin-coated surfaces enhanced attachment to certain types of cells (primarily epithelial cells, neurons, hepatocytes, and muscle cells).

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